Journal: Toxicological Sciences

Article Title: Microglia Activation and Gene Expression Alteration of Neurotrophins in the Hippocampus Following Early-Life Exposure to E-Cigarette Aerosols in a Murine Model

doi: 10.1093/toxsci/kfx257

Figure Lengend Snippet: Taqman Gene Expression Assays Used in RT-qPCR

Article Snippet: Quantified mRNA expression levels were calculated using the ΔΔCT method and normalized to the reference gene, glyceraldehyde-3-phosphate dehydrogenase ( Gapdh ). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Gene Gene Symbol Assay ID Amplicon Length Reference Sequence Nerve growth factor receptor Ngfr Mm00446296_m1 77 {"type":"entrez-nucleotide","attrs":{"text":"NM_033217.3","term_id":"118131161","term_text":"NM_033217.3"}} NM_033217.3 Brain derived neurotrophic factor Bdnf Mm04230607_s1 92 {"type":"entrez-nucleotide","attrs":{"text":"NM_007540.4","term_id":"114326462","term_text":"NM_007540.4"}} NM_007540.4 Glial cell line derived neurotrophic factor Gdnf Mm00599849_m1 101 {"type":"entrez-nucleotide","attrs":{"text":"NM_010275.2","term_id":"118129978","term_text":"NM_010275.2"}} NM_010275.2 Glyceraldehyde-3-phosphate dehydrogenase Gapdh Mm99999915_g1 109 {"type":"entrez-nucleotide","attrs":{"text":"NM_001289726.1","term_id":"576080554","term_text":"NM_001289726.1"}} NM_001289726.1 Open in a separate window Taqman Gene Expression Assays Used in RT-qPCR Serum cytokine/chemokine analysis Blood from the torso was collected from mice at the time of sacrifice, and serum was stored at –20 °C until analysis.

Techniques: Expressing, Amplification, Sequencing, Derivative Assay